BCHM 451 (cross listed as MBMB 451 and CHEM 451) is a two semester biochemistry course providing a thorough introduction to modern biochemistry for undergraduate and graduate students. BCHM 451a is devoted to structural biology, molecular biology, and cell biology, while BCHM 451b is devoted to enzymes, carbohydrate metabolism, membranes, lipid metabolism, amino acid metabolism, and nucleotide metabolism. Students should be familiar with basic concepts in organic and physical chemistry as well as quantitative approaches to problem solving.
Instructors:
(1) Blaine Bartholomew, bbartholomew@siumed.edu Neckers Building Room 211, phone 453-6437 Web site: http://www.siumed.edu/~bbartholomew/451A_Sec1.html (2) Peter Hardwicke, phardwicke@siumed.edu Neckers Building 210, phone 453-6469 Web site: http://www.siumed.edu/~phardwicke
COURSE OUTLINE:
COURSE OUTLINE:
First Section: Biomolecules
Chapter 1: Introduction
Chapter 5: Nucleic Acids, Gene Expression, and Recombinant DNA Technology
Chapter 29: Nucleic Acid Structures
Chapter 6: Techniques of Protein and Nucleic Acids
Chapter 7: Covalent Structures of Proteins and Nucleic Acids
Chapter 8: Three-Dimensional Structures of Proteins
Exam I Thursday September 18, 2008
Second Section: Gene Expression, Introduction to Thermodynamics
Instructor: Bartholomew
Chapter 31: Transcription
Chapter 34: Eukaryotic Gene Expression
Chapter 30: DNA Replication, Repair, and Recombination
Instructor: Hardwicke
Lectures 1 – 2: Outline of Principles of Thermodynamics.
Lecture 3 – 4: Ligand Binding. Dissociation and association constants. ‘Affinity’. ‘Saturation’. Important non-covalent forces in ligand and substrate binding and the folding of macromolecular. Simple ligand binding. Cooperative ligand binding. Agonists and competitive antagonists.
Exam II Thursday October 23, 2008
Third Section: Protein Structure/Function and Bioenergtics
Instructor: Hardwicke
Lectures 5 - 6. Water. Autoprotonation of water. pH. Strong acids, strong bases, weak acids, weak bases. Henderson-Hasselbach equation. Effect of H2O and H+ on DG.
Lectures 7-8. Amino acids. Isomers. Peptides. Isoelectric points (pI). Proteins. Forces involved in formation of protein structure. Primary, secondary, tertiary, and quaternary structure. Common motifs.
Lecture 8-10. Myoglobin and hemoglobin as examples of protein structure and ligand binding phenomena. Chapter 10 Voet and Voet.
Lecture 11-12 The Carbonic Acid - Bicarbonate Buffering System as An Example of pH Issues
MEETING TIME and PLACE:
Class meets twice a week on Tuesdays and Thursdays from 12:35-1:50 p.m in Life Science III Auditorium.
RESOURCES:
The primary text for this course is: Biochemistry by Voet and Voet. (Wiley Publishers, 2004, ISBN 0-471-19350-x)
Additional texts that you may find useful include:
Genes IX by Benjamin Lewin (Pearson Prentice Hall, 2007, ISBN-13: 978-0763740634)
Lehninger: Principles of Biochemistry Fourth Edition by Nelson and Cox. (W. H. Freeman and Company, 2005, ISBN 0-7167-4339-6)
It is always a good idea to consult as many sources as possible. There are many different ways to present the same material, and a different view can often be very enlightening. We highly recommend it!
EXAM SCHEDULE: all exams will be held in LSIII Auditorium
Exam I on Section I: Thursday September 18, 2008 (12:35-1:50 pm)
Exam II on Section II: Thursday October 23, 2008 (12:35-1:50 pm)
Exam III on Section III: Thursday December 4, 2008 (12:35-1:50 pm)
Final Exam (Comprehensive): Wednesday December 10, 2008 (12:50 p.m. - 2:50 p.m.)
GRADING POLICY:
Your performance in this course will be based on three exams plus a comprehensive final exam. Exams may consist of a combination of short-answer, multiple-choice, and problem-solving questions. Tests are scheduled to occur following seven to eight lectures. The lowest of the three exam scores will be dropped and each exam is worth 100 points. The comprehensive final exam is worth 200 points for a total possible of 400 points. The final exam will occur on the University's designated final exam day and time.
Letter grades will be assigned as follows:
A = 90 - 100%
B = 80 - 89%
C = 70 - 79%
D = 60 - 69%
F = 0 - 59%
Performance on problem sets and quizzes will be used to decide the grade for students whose cumulative scores fall on the borderline.
Comments and questions related to this web site should be directed to: bbartholomew@siumed.edu
SIUC/Molecular Biology, Microbiology and Biochemistry /URL: http://www.siumed.edu/~bbartholomew/BCHM451.html
Last updated: August 18, 2008/ bb