MBMB 451A Section 1
New Postings:Key for the 2nd exam is posted below. Also please check your grades at the link below using the four digit code that Dr. Hardwicke provided to you.
Old Postings:
Extra Credit: Write one review on one of the following topics using the indicated article:
(1) Nucleosome structure and dynamics click here
(2) Structure of eukaryotic transcription complexes click here
(3) Transcription regulation click here
The review article should be single spaced and be no longer han 5 pages.
A maximum of 15 points will be given on this assignment and will be used towards the 1st exam
The deadline for all extra credit to be turned in is October 22, 2008
Questions: How does RNA polymerase II find its correct binding site and is there such a thing as a sigma factor in this case? How does the activity of RNA polymerase II get modulated? How is transcription of particular genes get turned on in response to external stimuli such as stress (heat, starvation, and so on), hormones and other small molecule effectors? What is the difference between basal and activated transcription?
Click here for the home work that is due on Monday September 22, 2008
For Tuesday September 23, 2008 lecture: What causes chromatin to compacted into higher order structures? How does acetylation of lysine change the charge of its side chain? Why would this change affect the way histones might bind to DNA? Which amino acids are methylated? What is a HAT complex? Where can you find the simplest RNA polymerase in eukaryotes? What are the most conserved subunits of E. coli RNA polymerase and why do you think that is?
For September 4, 2008
Why are cDNA libraries still so important in the era of genomics? What information can be derived from cDNA that are not obtained generally by DNA microarrays?
Why would you need to purify a protein? What properties of proteins can be used to help separate and purify them from each other? What precautions should be taken in order to maintain the activity of your protein?
For September 2, 2008
Why are protein expression vectors useful? What kind of cells are proteins generally over-expressed in and why? What are the considerations in vector construction that need to be taken into account with over-expression of a protein?
For August 28, 2008
Find and be ready to discuss how DNA microarrays are used to study a particular problem (cancer, etc.). You will need to give specific examples. Some keywords that should help in you Google search are genome-wide expression profiling, cDNA microarrays.
Help answer the question of the effect in the Dideoxy (Sanger) Method of DNA sequencing of having different ratios of the dideoxynucleotide and the regular deoxynucleotide (i.e. ddATP vs dATP). Could this effect the range that can be sequenced and why? Review the principles of DNA footprinting - figure out why it is so important to cut the DNA only once instead of multiple times for this type of assay.
Course Information Professor and TA information Course Syllabus Course Material Study Guides Part 1 Part 2 Study Groups DNA Structure Supercoiling DNA methods Part 1 and Part 2 Molecular Cloning Protein methods Protein Structure Chromatin Structure Transcription Part 1 Transcription Part 2 Transcription Regulation Chromatin/Transcription 2007b; 2007; 2006; 2005; 2001 Exam II Key 2008 1st Homework 2nd Homework
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Last updated on October 28, 2008.